Quantification of Curdlan β-1，3-Glucooligosaccharides by Thin Layer Chromatography and Image Analysis

Abstract:

The study first found that a unidirectional-twice thin layer chromatography significantly improved the analysis of Curdlan β-1，3-glucooligosaccharides. With the best developing reagents acetic acid，ethyl acetate and H2O in the ratio of 2∶2.4∶1.4 on the Silica gel 60 F254 plate of 20 cm，Curdlan β-1，3-glucooligosaccharides could be separated from the degree of polymerization 2 to 13. Then，the Image J software was used to quantify Curdlan β-1，3-glucooligosaccharides. A good linear relationship（R2＞0.99） existed between saccharides concentration within three ranges of 0.02 g/L～0.1 g/L，0.1 g/L～0.8 g/L and 0.8 g/L～5.0 g/L and grayscale integration respond. The established quantification had a detection limit of 0.02 g/L and a relative standard deviation of 1 %～5 % with good stability. Compared with other methods such as HPLC，this method is simple and rapid with no specialized instruments and oligosaccharides standards.