Screening of Se-Enriched Probiotics and the Optimization of Se-Enriched Conditions

Abstract:

In order to obtain the Se-enriched probiotics strains which could tolerate high NaSeO3 concentration，7 strains of yeast and 12 strains of Lactobacillus were screened in this study. Results showed that all strains could grow on the plate with NaSeO3 concentrations of 20~80 μg/mL. Among them，strains of yeast FJYJM3 and Lactobacillus YQRS had higher tolerance and se-enriched ability. The optimization fermentation conditions showed that strain of YQRS had the highest se-enrichment when the addition concentration of NaSeO3 was 15 μg/mL and it was added at beginning of logarithmic phase. The biomass was 2.66 g/L and the total selenium content could reach to 2 300.26 μg/L. However，strain of FJYJM3 had the highest se-enrichment when the addition concentration of NaSeO3 was 20 μg/mL at beginning of logarithmic phase. Its biomass was up to 4.86 g/L and the total selenium content could reach to 5 790.99 μg/L.