Microwave-Assisted Enzymatic Transglycosylation and Hydrolysis of Stevioside

Abstract:

Microwave-assisted enzymatic transglycosylation and hydrolysis of stevioside （St） were investigated using α-cyclodextrin glucanotransferase （α-CGTase） and β-galactosidase，respectively. Microwave irradiation could accelerate the transglycosylation of St using α-CGTase extracted from Paenibacillus macerans JFB05-01，and the catalytic efficiency was increased by 21.7 folds. However，little effect was observed when microwave irradiation was applied to the enzymatic hydrolysis of St using β-galactosidase. With 1 000 U/g of α-CGTase was loaded，the conversion of St could reach as high as 71.6% after 1 min and the yield of St-Glc 1 was 23.7% correspondingly.