Screening and Optimizing Conditions of Chitinase Production for Bradyrhizobiaceae blastobacter SYBC-H2

Abstract:

Five strains of bacteria producing chitinase were separated from Jianmen harbour seafloor mud in Taizhou of Zhejiang Province，China. One of strains with higher enzyme activity was further identified by morphology，physiology and biochemistry，composition and content of fatty acid，content of（G+C） mol%，types of quinones，and molecular biological characteristics. The results showed that this strain belonged to the genus Blastobacter，named as Bradyrhizobiaceae blastobacter SYBC-H2. Moreover，the results of the Plackett-Burman Deisign experiments showed that chitin，glucose and corn steep powder were the key nutritional factors to produce chitinase. Composite Design Contral was applied to optimize the fermentative production of chitinase. As a result，the maximum chitinase activity was up to 5.70 U/mL when the culture medium was composed of 2.7 g/L chitin，0.85 g/L glucose and 2.64 g/L corn steep powder.