Expression and Purification of Zeaxanthin Glycosylase in E. coli

Abstract:

Several carotenoids are synthesized by related enzymes in Erwinia uredovora，. Zeaxanthin diglucoside is synthesized by zeaxanthin glycosylase which was encoded by gene crtX. In this study，crtX of E. uredovora was amplified by PCR and cloned into expression vector. The recombinant plasmid was then transformed into E. coli BL21 （DE3） to construct the engineering bacterium. The recombinant zeaxanthin glycosylase was purified by Ni2+ chelating affinity chromatography and a DEAE-Sepharose FF ion-exchange chromatography. The purified protein catalyze the formation of zeaxanthin diglucoside in vitro.