Effect of Low Temperature Domestication Speed on the Enzyme Acitivity in the Waterless Storage of Crucian

Abstract:

This work studied the impact of hyperthermia acclimation and storage temperature on waterless storage of live Crucian Carps. Crucian Carps were hyperthermia acclimated at the speed of 5 ℃/h and 1 ℃/h，and then cultured for 24 hours at 4 ℃ and 0 ℃ in waterless ice storage rooms to observe variations activity of metabolic enzyme and antioxidant enzyme. The results showed that the temperature adaption set as 1 ℃/h increased the activity of Lactate Dehydrogenase（LDH）. The activity of isocitrate dehydrogenase（IDH） was maintained comparatively well under such condition，while brain succinate dehydrogenas （SDH） didn't change. Alaninetraensaminase （ALT） activity was maintained at 0 ℃storage，and Malondialdehyde（MDA） acitivity was accumulated at 0 ℃. No obvious change was discovered in liver，which was caused by accumulated Catalase（CAT） low temperature storage.