Mutation Breeding and Cordycepin Production Study of Cordyceps militaris JN168

Abstract:

Cordycepin is the major effective component of Cordyceps militaris，which is getting more and more attention for its hygienic and medical effects. In this study，we obtained several strains with high cordycepin yield from Cordyceps militaris JN168 by using ion beam，nitrosoguanidine and ion beam-nitrosoguanidine mixed mutation. After further screened，the high yield compound mutation strain 2 was obtained. Furthermore，the liquid medium composition of this strain was optimized for the fermentation of cordycepin. The results showed that the components of optimized medium were as follows：glucose 40 g/L，yeast extract 25 g/L，MgSO4·7H2O 0.6 g/L，K2HPO4·3H2O 0.6 g/L，KH2PO4 0.6 g/L. With this optimized medium，the yield of cordycepin increased 5 times，reaching 1 045.65 mg/L.