High Sensitive Detection of Sulfadimethoxine Based on SPR Technique

Abstract:

The overdose of sulfa drugs will cause allergic reactions and possibly carcinogenic. Among the all sulfa drugs used in food，sulfadimethoxine(SDM) takes up a large proportion. In this research，a new method based on surface plasmon resonance(SPR) was proposed for the rapid and high sensitive detection of SDM in milk powder. The detection was based on the changes of SPR angles，which was subsequently caused by the changes in the refractive index of gold film induced by the specific recognition of samples by the SDM antibody modified on the surface of SRP chips. A standard curve represented the relationship between SRP response signal and SDM concentration. The limit of detection(LOD) for SDM was as low as 0.19 ng/mL，and the milk powder recovery was in the range of 94.0%～98.3%. Compared with the traditional methods，SPR technique has high sensitivity，high accuracy and easy operation. SPR sensor chips can be repeatedly used for sample detection in practical application with good integrity.