Sulfated Modification of Extracellular Polysaccharide from Submerged Fermentation of Ganoderma lucidum

Abstract:

The extracellular polysaccharides from submerged fermentation of Ganoderma lucidum exhibit low anticancer activity. Sulfation of polysaccharides is an important approach to improve anticancer activity of polysaccharides. In addition, the degree of sulfated substitution (DS) is closely related to its biological activities. In order to understand the relationship between DS and sulfated conditions and obtain the sulfated derivatives with different DS, the sulfuric acid esterification reagent and the relationship between the sulfated reaction conditions and DS were further investigated. The results showed that aminosulfonic acid offered milder reaction conditions than that of chlorosulfonic acid. On the other hand, the addition of urea also gave mild reaction conditions and improved DS.