Two-Stage Temperature Controlling Strategy For Improving the Yield of Expolysaccharide

Abstract:

The fermentation production of expolysaccharide from Cordyceps militaris was studied at different temperature. The results showed that the optimal growth temperature was at 26 ℃ and the highest yield of expolysaccharide accumlation temperature was at 28 ℃. Thus,the two-stage temperature control strategy was developed based on RSM. When pre-fermentation temperature was kept at 26 ℃ for 17 hours and changed to 28.5 ℃ ,the yield increased to 10.92 g/L which was 25.5% higher than that of fermentation at single temperature at 28 ℃.