Separation, Purification and Antioxidant Activity Study of Antioxidant Peptides from Pleurotus ferulae lenzi in Xinjiang

Abstract:

Antioxidant peptides of Pleurotus ferulae lenzi were separated using ultra-filtration,ion-exchange and gel filtration chromatography,and its antioxidant properties were also studied in this study. Two kinds of peptides(＞10 000 polypeptide components and ＜10 000 polypeptide components )were isolated from the hydrolyzed solution of Pleurotus ferulae lenzi protein. The polypeptide components under 10 000 have higher ·OH radical scavenging and DPPH· radical scavenging capacity than those above 10 000. After detaching of polypeptide components under 10 000 by DEAE-32,three elution peaks were obtained and P3 component showed higher hydroxyl scavenging capacity,reaching 50.22%. Five elution peaks at 220 nm were further isolated from P3 component by Sephadex G-25,and P3-2 component showed best capacity of ·OH radical scavenging,reaching 77.23%. According to standard equation,the molecular weight of P3-2 was about 1 862. The results provide a theoretical basis for further studies of active ingredient from Pleurotus ferulae lenzi in Xinjiang province.