Abstract:Hyriopsis cumingii visceral organ and Nacl solution were used as material and extraction liquor to study the hyriopsis cumingii glycoprotein extraction and separation under low temperature and the antioxidant activity. Through single factor experiment and orthogonal experiment,the optimized extraction condition was as below:extraction temperature at 4 ℃,0.3 mol/L NaCl solution,solid-liquid ratio in 1∶15,and extraction for 9 h. Glycoprotein extraction rates under optimal condition interms of sugar content and protein content were (9.259±0.083)% and (3.763±0.107)% respectively. Crude glycoprotein sample,that comprises three different components,was obtained,after the extraction liquor was fractionally precipitated by ammonium sulfate. The different component had a different ability to scavenge surperoxide anion radicals and hydroxyl radicals. Crude glycoprotein sample was Electrophoresed by 10%SDS-PAGE and dyed by Coomassie Brilliant Blue R250 and PAS,and the result showed the three components contained different glycoprotein bands.
