Antioxidative and Cholesterol-Rducing Ability of Weissella viridescens ORC4 from Dry Fermented Sausages

Abstract:

A Gram(＋),non-motile,facultatively anaerobic,rod-or coccoid-shaped bacterium,designated strain ORC4,was isolated from the dry fermented sausages. The 16S rRNA gene sequence analysis showed that strain ORC4 belonged to the genus Weissella,with highest sequence similarity to Weissella viridescens NRIC1536(100%). The intact cells and intracellular extract of strain ORC4 could inhibit O2-·,DPPH·,and ·OH. Three free radicals-scavenging ratios of intracellular extract were positively correlated with bacterial density,respectively,the intact cells could do the same. The intracellular extract had higher scavenging ratios on three free radicals than the intact cells. At a concentration of 5×108 CFU/mL,scavenging ratios of intracellular extract on O2-·,DPPH· and ·OH were 45.17%,56.17% and 54.01% respectively. In addition,strain ORC4 could reduce 35.87 μg/mL cholesterol,and the unit cell dry weight of cholesterol removal rate was 19.89 μg/mg.