Application of Debranching Enzyme for the Extraction of Rice Protein

Abstract:

Debranching enzyme was used to enhance the extraction efficiency and product quality of rice protein. A significant decrease in molecular weight was detected by HPSEC for rice starch digested by debranching enzyme and the 1H-NMR analysis proved that the enzyme completely hydrolyzed α-1,6-linkages, which facilitated the following function of glucoamylase. Debranching enzyme together with α-amylase and glucoamylase could entirely hydrolyze the dextrin in liquefied rice dreg after sugar processing into water soluble oligosaccharides, which increased the removal rate by 38.9% compared with the processing only through glucoamylase. The total nitrogen content of the crude protein reached up to 90.58%, which was higher than that obtained by the traditional methods.