Molecular Authentication of Panax ginseng and Panax quinquefolius by their Ribosomal DNA External Rranscribed Spacer Region

Abstract:

Panax ginseng and Panax quinquefolius are two important traditional Chinese medicine with similar morphology but different medicinal efficacy. This study aimed to develop a simple and effective analysis of molecular authentication of Panax ginseng and Panax quinquefolius. Two allele-specific primers of Panax ginseng and Panax quinquefolius were designed from their Single Nucleotide Polymorphism (SNP) created by ribosomal DNA (rDNA) external transcribed space (ETS). Multiplex polymerase chain reaction (Multiplex PCR) was conducted for identification of Panax ginseng and Panax quinquefolius. The results confirmed the effective authentication of Panax ginseng and Panax quinquefolius as well as their mixture by the established PCR method.