Synergistic Effect of Feruloyl Esterase and Cellulase on the Release of Ferulic Acid from Wheat Bran

Abstract:

The synergistic effect of feruloyl esterase and cellulose on the release of ferulic acid from de-starched wheat bran (DSWB) was studied. The HPLC results showed that DSWB which was ultrasonically pretreated at a power of 350?W for 20 minutes released ferulic acid at a level of 1.45-fold more than that unpretreated. ReAoFaeA (30 U) by itself released only 4.1% of total alkali-extractable ferulic acid from wheat bran, while no ferulic acid released if rAuCel12A was used. The rate of ferulic acid released was significantly increased when both enzymes were used. The conditions using two enzymes simultaneously were optimized by a single factor test. A maximum of 23.6% total ferulic acid released could be reached in combinations of 30 U reAoFaeA and 70 U rAuCel12A with the ratio of solid to liquid of 1∶30 (g∶mL) at pH 5.0 and treated under 40 °C for 10 h.