Study on the Biodegradation of Pyridine by Lactobacillus Reuteri from Camel Rumen Extracts

Abstract:

8 lactobacillus reuteri were isolated from camel rumen and then cultured in mineral saults medium using pyridine (300 μg/mL) as the sole carbon source and nitrogen source. The absorbance at 600 nm was periodically measured by UV spectrophotometer to check the growth of Lactobacillus. The degradation of pyridin by lactobacillus reuteri was dynamically detected using Gas Chromatography-Mass Spectrometry (GC-MS) and the residual amount after degradation was calculated. According to the results, all the lactobacillus could effectively degraded pyridine, among which GU366027 and GU366021 can completely degraded pyridine when cultured for 84 h, while GU366034, GU366019 and U366030 needed 96h and others (GU366037, GU366038, GU366028) for 108 h.