Biological Preparation of CDP-C by Issatchenkiaorientalis CCTCCNO:M2011272

Abstract:

5'-cytidine acid and choline calcium phosphate as the substrates were converted into CDP-C by Issatchenkiaorientalis.The optimal conversion condition was as follows:CMP17.5 g/L,choline calcium phosphate 12.5 g/L,potassium hydrogen phosphate 12.5 g/L,seven hydrated magnesium sulfate 3.0 g/L,manganese sulfate 0.6 g/L,glucose 50 g/L,toluene 30 mL/L,Yeast mud 375 g/L,and the pH of convert liquid 8,the output of CDP-C can reach more than 10 g/L,up to 11.592 g/L. The conversion solution was decellularized,deproteinized and the CDP-C was obtained by eluting 717 ion exchange resin with 0.05 M NaCl,whose purity was more than 98%.