Effect of GABA on RIN-m5f Cells in High Glucose－Induced Oxidative Injury Model

Abstract:

Oxidative injury was induced by high glucose. Cell viability,production of ROS,the activity of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px),malondialdehyde(MDA) content,insulin secretion and related genes level were measured to investigate the effect of different concentration of GABA(10、50、100 μmol/L) on RIN-m5f cells in high glucose-induced oxidative injury model. The results demonstrated that GABA improved the cell viability,reduced ROS ptoduction,significant enhanced the activity of SOD and GSH-Px,lowered the MDA content,ptomoted insulin secretion,increased the mRNA level of Nrf2 and Bcl-2,and decrease the mRNA level of GSK-3β and Bax. It follows that GABA possesses the anti-apoptosis and anti-oxidative protective effect on RIN-m5f,and this ptotection may be achieved by inhibit GSK-3β and then inhibit Bax,and activate several key factors such as Nrf2 and Bcl-2.