Isolation and Identification of Endophytic Bacteria in Paris polyphylla Smith var．yunnanensis (Franch) and Phylogenetic Analysis

Abstract:

In this manuscript,the endophyte of Paris polyphylla Smith var．yunnanensis(Franch) was isolated and identified. 40 and 44 endophytic bacteria were isolated from the root of cultivated or wild Paris polyphylla Smith var．yunnanensis in Wenshan,Yunnan province. They were then identified and categorized as 6 and 8 genera by 16S rRNA gene sequenceing. Bacillus and Enterobacter were dominant genera in cultivated of Paris polyphylla Smith var．yunnanensis because they accounted for 35% and 27.5% respectively in the identified bacteria;Bacillus and Klebsiella were dominant genera in wild Paris polyphylla Smith var．yunnanensis and accounted for 36.4% and 20.4% respectively. The results reflected the difference and biodiversity of endophytic bacteria between cultivated Paris polyphylla Smith var．yunnanensis and wild ones. This study provides theoretical and experimental basis for subsequent development of Paris polyphylla Smith var．yunnanensis.