Study on Extraction Technology of Limonin from Citrus Changshan Peel

Abstract:

To develop a sensitive high performance liquid chromatography(HPLC)method to determine Limonin in Citrus Changshan peel. Different extraction technologies were compared in this study. There are three methods which were selected：organic solvent extraction, water extraction and ultrasonic extraction. The optimum conditions of ethanol extraction were achieved as follows：soaking temperature as 50 ℃, the ratio of materials to solution as 1∶20(g/mL), extraction time as 60 min. The Limonin content of Citrus Changshan peel was 2.97 mg/g. The optimum conditions for water extraction were achieved and listed as follows：soaking temperature as 80 ℃, the ratio of materials to solution as 1∶25(g/mL), extraction time as 90 min. The Limonin content of Citrus Changshan peel was 2.42 mg/g. The Limonin content of Citrus Changshan peel with ultrasonic extraction was 3.32 mg/g by ethanol and 2.87 mg/g by water.