Inhibitory Effects of Extract Fraction 2 from Antrodia cinnamomea on the Activation of CFSC-8B Cells Induced by PDGF-BB

Abstract:

This research aimed to investigate the effects of extract fraction 2(Fr.2) from Antrodia cinnamomea on the rat hepatic stellate cells(HSCs). A cultured rat HSC line CFSC-8B was stimulated by PDGF-BB in the presence or absence of Fr.2. Cell proliferation, cell migration, extracellular matrix(ECM) production and anti-fibrotic mechanism were studied. We found that CFSC-8B cell proliferation could be induced by 10 ng/mL PDGF-BB for 48 h. The IC50 value of Fr.2 was 56.46 μg/mL. Fr.2(25 μg/mL) significantly inhibited CFSC-8B cell proliferation and migration induced by PDGF-BB. Fr.2 also decreased α-SMA, Col 1, Col 3 and Fn mRNA expression. Mechanistically, Fr.2 negatively regulated p-ERK, p-JNK and p-P38 expression. Therefore, we provide evidence to demonstrate Fr.2 from A. cinnamomea inhibited HSCs proliferation, migration and ECM production induced by PDGF-BB, which in part, through negatively regulates MAPK signaling.