Screening of Alginate Lyase Producing Strain Cobetia sp. WG-007 and Optimization of Fermentation

Abstract:

A high efficient alginate-degrading strain was isolated from rotten seaweed using sodium alginate as the sole carbon source. Based on the physiological characteristics as well as 16S rDNA sequencing, the strain was identified as Cobetia sp. WG-007. The optimized fermentation medium was composed of sodium alginate 6.0 g/L, peptone 5.0 g/L, NaCl 20 g/L, K2HPO4 0.25 g/L. The strain was cultivated at 25 ℃ for 30 h under the optimized conditions and the alginate lyase activity was increased to 160.7 U/mL, which was 3.52-fold compared with the control.