Abstract:In order to improve the antibacterial effect of lysozyme on Escherichia coli and other gram negative bacteria, according to the single-factor experiments and orthogonal methodology was employed to optimize the Iodoacetamide modified lysozyme. The results show that 0.06 mol/L iodine acetamide and 5 mg/mL lysozyme bacteriostatic effect is best. The biggest inhibition zone diameter to the Escherichia coli is 25.7 mm when the reaction temperature is 45 ℃, reaction time is 30 min, and pH is 5. The antibacterial circle diameter is 2.7 times of the original. The biggest inhibition zone diameter to the salmonella is 24.1 mm when the reaction temperature is 55 ℃, reaction time is 70 min, and pH is 6. The antibacterial circle diameter is 2.6 times of the original. This was considered that egg white lysozyme after iodoacetamide modified, can be used for Gram-negative bacteria (E.coli K12, salmonella) inhibition, and the effect is good.
