Abstract:The optimization of the ultrasonic-assisted extraction of total alkaloids from Plumula Nelumbinis by response surface methodology was studied. Based on the single-factor test analysis, the Plackett-Burman experimental design and steepest ascent design, while take extracted amount of total alkaloids as the response value, response surface method was applyed to analyze the influence of various factors and their interaction on the extraction of total alkaloids, select an optimized progress to extract total alkaloids from Plumula Nelumbinis. In this study, nonlinear mathematical model fitting way make independent variables and the dependent variable relationship extend to curved surface, so that the experimental data become more precise. The experiments obtain an optimal ultrasound-assisted extraction of total alkaloids from Plumula Nelumbinis progress conditions: solid-liquid ratio as 1∶40.4; volume fraction of ethanol as 64.4 %; ultrasound extraction time as 40 min; model predict its productive yield can reach 128.695 mg/g. Under these conditions, extracted amount of total alkaloids from Plumula Nelumbinis can reach 128.673 mg/g, which is consistent with the predicted values.
