Abstract:Phosphatidylinositol(PI) signaling system was critical in many plant developmental processes including seed development, growth and reproduction, aging and cell responses to environmental stress. Based on the construction of the transcriptome library and the expression profile of Camellia oleifera seeds both in the fruit expanding period and at the peak stage of lipid biosynthesis and oil synthesis, the key enzyme genes related to PI signaling system has been analyzed in detail. The research suggested that there are 212 non-redundant gene unigenes related to PI signaling sistem of Camellia oleifera seeds which mainly encode 12 kind of key enzyme proteins such as PIK, PIPK, PIS, DGK, PLC et al. The results of expression profile analysis showed that these genes have different expression patterns in different developmental stage of Camellia oleifera seeds. The findings will be in favor of the research of interaction between IP pathway and other signaling pathways and provide a scientific basis for Camellia oleifera breeding of good quality.