Ubiquitination Detection of NCR Related Permeases and Regulators

Abstract:

To give support to release the nitrogen catabolite repression(NCR), by rebuiling the ubiquitination system, the ubiquitination of NCR related permeases and regulators was detected. A fast ubiquitination detection procedure established before was modified and developed, which enhanced the fluorescence of the positive control and could quantify the fluorescence signal. The fluorescence quantification made this procedure more reliable. It was demonstrated that 5 of the NCR related proteins were ubiquitinated, 4 were not ubiquitinated and 1 was not determined. We not only established a reliable ubiquitination detection procedure, but also gave a idea to release NCR through the ubiquitination modification procedure.