Abstract:In order to obtain antioxidative peptides from Spirulina platensis,this study was performed to optimize the enzymatic hydrolysis process of Spirulina platensis by papain and to purify antioxidant peptides from digests of Spirulina platensis by ultra-filtration and gel filtration chromatography,and the antioxidative activity was also determined in vitro. The results indicated that the optimum hydrolysis conditions of papain were given as follows:ratio of enzyme to substrate 1.6%,temperature 60 ℃,hydrolysis time 9 h,pH 7. The hydrolysates with molecular weights ranging from 0 to 3 000 had the most potent antioxidative activity. This fraction of the hydrolysate was purified by gel chromatography to obtain antioxidative peptides with higher activity. The DPPH radical scavenging rate,hydroxyl radical scavenging rate and total antioxidant capacity of the purified antioxidative peptides were equivalent to those of(1.01±0.07)×10-2mg/mL,(4.77±0.28)× 10-1mg/mL and(3.79±0.81)×10-2mg/mL ascorbic acid solution respectively,suggesting that the antioxidative peptides from Spirulina platensis possess high antioxidative activity.
