Formation Mechanism of Tetramethylpyrazine Produced with B.subtilis S12 Under the Fermentation Condition Simulated Bacterial Qu Preparation Used for Chinese Liquor Brewing

Abstract:

Tetramethylpyrazine(TTMP) also known as 1igustiazine,is a key flavor of Chinese liquor. To clear the formation mechanism of TTMP in Chinese liquor,the formation mechanism of TTMP in bacterial Qu was explored with a single strain of B. subtilis S12 under simulated conditions of bacterial Qu preparation. The results revealed that the synthesis of TTMP can be divided into two stages:the 3-hydroxy-2-butanone and ammonia are mainly synthesized by B. subtilis S12 firstly;then the acetoin and ammonium ion undertake chemical reaction and produce 2,3,5,6-TTMP under high temperature in the thermal power stage. The results signified that high temperature was favorable for increasing TTMP production.