Production of Catalase Based on Controlling of Key Factors

Abstract:

The effect of different key factors on production of catalase(CAT) in recombinant Escherichia coli was analyzed by orthogonal experiment. The optimum fermentation condition in shake flask was determined:glycerol 5 g/L,yeast extract 35 g/L,initial pH 7.5,and induction temperature 30 ℃. During production of CAT in 3 L fermentor, the highest activity reached20 117.2 U/mL at the optimum stirring speed(300 r/min). The optimum initial glycerol concentration was 10 g/L. The highest activity reached 28 243.0 U/mL when the speed of glycerol supplement was0.67 g/(L·h). When 16.7 g/L glycerol was supplemented at start induction,the CAT activity could reach 50 369.5 U/mL after incubation for 47 h,which was 2.5 fold of that before optimization.