A Nanoparticle-Enhanced ELISA Against Nortestosteron in Animal-Original Food

Abstract:

Conjugating enzyme with nanoparticles is an effective approach to enhance the characteristics of enzyme molecule in biosensor.A highly active enzyme-nanoparticle complex,HRP-IgG GNP,was prepared by immobilizing horseradish peroxidase labeled antibody(HRP-IgG) onto the surface of gold nanoparticle(GNP).The HRP-IgG GNP complex was applied to the enzyme-linked absorbent immunoassay(ELISA) and a highly sensitive nanopaticle-enhanced ELISA method was developed against nortestosterone(NT).The sensitivity of the novel method reached 0.01 ng/mL and the linear range was 0.03 ~10 ng/mL.the nanopaticle-enhanced ELISA method was 10 times more sensitive than the traditional ELISA method.The above method has promising applications for developing highly sensitive immunoassay methods.