Studies on the Protein Fingerprint of Salmonella by MALDI-TOF-MS

Abstract:

Using Salmonella enterica DSM 17058 T as the research model,the protein fingerprint of Salmonella was studied to obtain an accurate identification result based on MALDI-TOF-MS.In order to achieve a good reproducibility of the protein map and establish the standard analysis method by MALDI-TOF-MS,different mediums,culture times and sample handing methods were optimized.The results showed that more characteristic peak of protein with smooth baseline,less noise and higher signal-to-noise ratio were obtained by ethanol/formic acid treatment method.Taking Columbia Agar Base as the culture medium,the protein map with strong signal and good repeatability was acquired after 24h,which took less time compared with other mediums.