Screening and Decolorization of Malachite Green of a Manganses Peroxidase-Producing Bacteria

Abstract:

Six strains with manganses peroxidase-producing capability were screened from the soil samples which were collected in a Wood factory,Wuxi,China.The strain J09 which has the highest yield was identified as Sinorhizobium meliloti.The enzyme activity reached the peak of 607.7 U/L at the third day under the optimized conditions.For the crude enzyme liquid and fermentation medium with initial concentration of 15 mg/L malachite green,the decolorization rate could reach more than 75% and 85% by 3 hours respectively.Under aerobic condition the decolorization rate was higher than that under the anaerobic condition and malachite green has some toxicity to the bacteria.