Application of Mass Spectrometry in Amino Acids Analysis

Abstract:

Amino acid analysis is used to determine the amino acid content of amino acid-,peptide-and protein-containing samples.In the last decades,amino acid separations have been carried out mainly by ion-exchange chromatography with post-column derivatization and RP-HPLC with pre-column derivatization.Along with the modern technique of amino acid analysis,high throughput and sensitivity mass spectrometry has been widely used in the analysis of amino acids and brings significant benefits.This review focus on the application of the gas chromatography,liquid chromatography and capillary electrophoresis in combination with mass spectrometry in the analysis of amino acids,for which comparison are made.