Isolation,Screening and Identification of Endophytic Actinomycets from Taxus Chinensis

Abstract:

In this manuscript,endophytic actinomycetes were isolated from the root,stem and leaf of Taxus chinensis by the method of tissue homogenate.Antimicrobial activities of endophytic actinomycetes fermentation broth were determined with filter paper and molecular identification of some potential strains were also carried out.The result indicated that 55 strains of endophytic actinomycetes were isolated,21 of them have varied antimicrobial activity,accounting for 38.2% of all strains and 4 strains have higher antimicrobial activity.Results of 16S rDNA gene sequences analysis of the 4 strains showed that 3 of them belong to Streptomyces sp.,1 belongs to Mciromonospora sp..