Preparation and Immunological Characterization of Monoclonal Antibody Against Melamine

Abstract:

In this study,mice BALB/C were immunized by melamine-BSA and anti-melamine monoclonal antibody(McAb) was prepared by using hybridoma technique.The ELISA titers of ascite was exceeded 50,0000.Subtype of the McAb was IgG1 and the affinity constant was 3.21 109 L/mol.The cross-reactivity with Cyanuric acid,cyanurodiamide and ammelide was 30%,93% and 46% respectively.The monoclonal antibody can be used for fast determination of melamine substances.