Abstract:The freshwater fish were selected as materials,and a HPLC-RI method for detecting reducing sugars,phosphorylated reducing sugars and sucrose from fish meat and other foods was established in this study.The chromatographic conditions of reducing sugars were investigated and optimized.A Hypersil NH2(4.6 250 mm,2.5 μm) column was used,the mobile phases were 80% ethanol and 20% purified water at the flow rate of 0.6 mL/min,the column temperature was 25 ℃,and the differential refractive index detector temperature was 40 ℃.Ethanol/water(80% v/v) was used to extract mono-and disaccharides from dorsal and belly of fish,and adding resin to remove the amino acids and salts from the extract.The sugars in fish dorsal and belly were determined by the established HPLC-RI method.Phosphorylated sugars were first dephosphorylated by alkaline phosphatase and then determined by the same method.The result showed that the method had a good resolution and good linearity,the recovery rate was between 70.2% and 98.5%,and the RSD of repeatability were all lower than 5%.The result of detection showed that there were differences in category and content of sugars between different parts of fish.
