Maillard Reaction between Chitosan Oligosaccharide and Glucose/Maltose and the Antioxidant Activity of the Derivatives

Abstract:

Maillard reaction was occured by heating chitosan oligosaccharide(COS) with glucose and maltose separately(the ratio of —NH2 and —OH was 3∶1).The absorbance and fluorescence were determined during the reaction.Two kinds of chitosan oligosaccharide derivatives were extracted,named CG and CM.Chitosan oligosaccharide derivatives were characterized by FTIR and their molecular weight was determined by GPC.Their antioxidant activity was investigated by employing various established in vitro systems,such as superoxide/1,1-diphenyl-2-picrylhydrazyl(DPPH) radicals scavenging and reducing power.The results showed two kinds of the chitosan oligosaccharide derivatives showed strong antioxidant activity compared with chitosan oligosaccharide.The antioxidant activity was found to be in the order of CG>CM>COS,which indicated that the derivatives prepared by monosaccharide had the better antioxidant activity.