Abstract:The aim of this manuscript is to prepare the catalase-loaded solid lipid nanoparticles using the W/O/W double emulsion and solvent evaporation techniques.The results showed that PLGA was an efficient emulsifier for the preparation,the concentration of PLGA had an effect on the particle size and zeta potential.From the results we can conclude that the sonication time for the W/O emulsion was 20 s,the sonication time for the W/O/W double emulsion was 30s,Methylene chloride/acetone(1/1) was selected as the organic solvent,the concentration of PLGA was 15%,and the encapsulation efficiency was achieved at 58.75%,also we got the homogeneous particles.The vitro release results showed Higuchi equation could apply to this release curves.This results presented here indicated that the carrier can prolong the action time of the catalase.The CAT structure also was not damaged during this process through determining the activity.
