Studies on the Extraction and Distribution of the Enzymes in the Thermophilic Anaerobic Digestion System of Cassava-Ethanol Wastewater

Abstract:

The main components of the solid content in the cassava-ethanol wastewater were cellulose and hemicellulose,which account for about 30% and 18%,respectively.Because the endo-β-1,4-glucanase(CMCase) and xylanase played a key role in hydrolyzing cellulose and hemicellulose during the thermophilic anaerobic digestion process,six methods for extracting CMCase and xylanase from the anaerobic sludge were compared and the distribution of these enzymes in the sludge was also investigated in this study.The results demonstrated the extraction of CMCase and xylanase using 1% Triton X-100 was an efficient and gentle method,meanwhile,at least 90% of CMCase and 70% of xylanase were immobilized on the flocs(the surface of cells or the extracellular polymeric substances).