Progress in Cryopreservation of Microorganism in Liquid Nitrogen

Abstract:

Culture collection is a basic research work in microorganism.Cryopreservation,transfer of biological objects to deep cold anabiosis(-196 ℃),is an efficient method for long term storage of microorganisms.In this review,the parameters important for development of efficient technologies for cryopreservation of lactic acid bacteria,yeast,microalgae and protozoa are addressed.Furthermore,the problems in liquid nitrogen cryopreservation are discussed in this article.