An Efficient Method for Glutathione Extraction from Dried Saccharomyces Cells

Abstract:

An efficient method for glutathione(GSH) extraction from the dried Sacchromyces cells was proposed in this study.During extraction at(70±1)℃,the mixture of acetic acid-hydrochloric acid was used to adjusted pH 3.5,the ratio of solid to liquid(w/v) was kept at 1:8. GSH extraction result reached at 5.5 mg/g,which was 54.49%-62.24%higher than that of all of the reported extraction process.After treatment through micro-filtration(0.45、0.22μm) and ultra-filtration(5 kD MWCO),60.98%of the yeast protein was removed.The collection from ultrafiltration was analyzed by high performance liquid chromatography and amino acid analyzer, the result showed that glutamic acid was the major portion in amino acids impurity besides protein and small peptide in crude extract,and these would provide basic data for purifying GSH.