The Polymorphism of Lactobacilli from Acid Whey for Dairy Fan in Yunnan Area

Abstract:

The target of this study is to investigate the biodiversity of 70 Lactobacilli strains,isolated from the acid whey for dairy fan made by herd man families in Yunnan Dali the Bai nationality Region in China,for this,AFLP technique integrated with NTSYS-pc2.1 software was developed to investigate the polymorphism and types of the strains.The results suggested that the amplified bands of AFLP were clear and stable with high repetition and high polymorphisms.The cluster analysis showed that at level 0.89,70 tested strains were clustered into 4 groups: Lactobacillus fermentum,Lactobacillus helveticus(65.7%) and Lactobacillus plantarum.The biodiversity of isolates from Ⅲ was further investigated.At level 0.90,they were clustered into 8 genotypes.