Abstract:OBJECTIVE To investigate the protective effect of Rosa roxburghii Tratt juice(100% raw juice)and Rosa roxburghii Tratt collagen drink on restraint-stress induced liver injury of mice.METHODS The male KM mice were randomly divided into normal control group,stress model group,Rosa roxburghii Tratt juice group and Rosa roxburghii Tratt collagen drink group.The mice were orally administered samples once a day successively for 7 days,then restrained for 18 h after 30 min of the last intragastric administration.Datum were obtained including activities of Alanine aminotransferase(ALT) and the Oxygen radical absorbance capacity(ORAC)value in plasma,the content of Malondialdehyde(MDA),glutathione(GSH) and activities of superoxide dismutase(SOD) in liver.Antioxidant capacity of Rosa roxburghii Tratt juice and collagen drink in vitro were also determined.RESULTS Rosa roxburghii Tratt juice and collagen drink can markedly decrease activities of ALT in plasma,reduced MDA level in liver and effectively increase ORAC value in plasma of mice compared with the model group.They can also increase SOD activities in mice liver.Apart from the above roles,Rosa roxburghii Tratt collagen drink can increase GSH content in mice liver compared with model group.Additionally,Rosa roxburghii Tratt juice and collagen drink can effectively increase the ORAC value in vitro.CONCLUSION The results indicate that Rosa roxburghii Tratt and collagen drink had the effect of protecting liver from damage induced by stress in mice.It is considered that this protective effect may be related to its free radical scavenging activity and lipid peroxidation inhibitory effects.
