A Study of Immunoenzymatic Techniques of the Adsorption Effect of Nano-Gold to Antibody-Fc Part

Abstract:

In this study,a nano-gold which diameters were about 15 nm was prepared through a the simple and rapid microwave technique.The nano-gold absorbing rabbit IgG was performed by UV-visible spectrophotometer.The absorption maximum wavelength of gold colloidal solution was obvious changed when IgG coupled to nano-gold.It range from 518 nm to 526 nm.For the adsorption effect of nano-gold to antibody-Fc part,it was described by the variation of O.D.When rabbit IgG reacted with HRP-labeled goat anti-rabbit IgG and formed a compound,the remaining HRP-labeled goat anti-rabbit IgG in the supernatant decreased after centrifugation.The HRP catalyzed substrate reaction with the color variation.Finally it was shown a good adsorption effect through the ratio of O.D,and the percentage was over 92%.