Abstract:In order to isolate a tacrolimus producing strain,soil samples were initially treated with 75 μg/mL K2Cr2O7 and 2 μg/mL benzylpenicillin.Seventy-one actinomycetes were isolated by anti-fungal activity against Candida albicans then the metabolites origin from those isolations were further analysis by HPLC.Among these,only one tacrolimus producing strain was determined.It was identified by culture characteristics,morphological characteristics,physiological,biochemical properties and 16S rDNA sequence and phylogenetic analysis.The screened strain was gram-positive,the growth temperature ranged from 15 ℃ to 38 ℃(the optimum was 28 ℃);the growth pH ranged from 6.5 to 8.5(the optimum at 7.0).The strain can grown on glucose,sucrose,maltose,xylose,fructose,lactose and rhamnose.Based on the results of the general taxonomical characteristics and the 16S rDNA sequence,the isolated has proven to be Streptomyces and named Streptomyces sp.NJYH2618.
