Abstract:The obective of this study is to isolate mangrove forest derived actinomyces strains for obtaining the bioactive metabolite producing strains to investigate antifungal and anti-tumor active metabolites.For this,actinomyces strains were isolated by single colony isolation technique.The antifungal activity was assayed by paper method.The anti-tumor activity was assayed by MTT method.Ninety-five strains of actinomyces were isolated from 3 soil samples and 2 water samples collected from a mangrove forest in Futian,south of Shenzhen,China,of which 94% were streptomycetaceae.Nine pathogens were used to test the antifungal activity of the ninety-five strains,among of them 31.6% of the fermentation broth showed the antifungal activity.Two tumor cell lines(A549 and K562) were used to test the antitumor activity with the MTT method.The result indicated that 21% of the fermentation broth presented the antitumor activity.Furthermore,the fermentation broth of the s33,s62,s65and s68 showed antifungal activity toward more than five pathogens and antitumor activity on the two cell lines.
