Abstract:The objective of this study is to investigate scavenging activity of tetramethylpyrazine(TMP),an effective constituent isolated from the rhizome of nature medicinal plant Ligusticum chuanxiong Hort.,on free radicals in vitro and inhibitory effect of TMP on H2O2-induced oxidative damage of endothelial cells.The inhibition of H2O2-induced intracellular reactive oxygen species(ROS) by TMP was analyzed on a FACS Vantage SE flow cytometer.The suppressive effect of TMP on nitric oxide(NO) production was measured by Griess method.The scavenging effect of TMP on free radicals including hydroxyl radical(·OH),hydrogen peroxide(H2O2),DPPH free radicle(DPPH·) and lipid peroxidation free radical was determined by colorimetry method.It was found that TMP inhibited H2O2-induced production of intracellular ROS and exhibited strong scavenging activity against hydroxyl radical.In addition,TMP displayed scavenging activity against DPPH·,NO,H2O2 and lipid peroxidation.The results presented here indicated that TMP may exhibit scavenging activity on free radicals in vitro and inhibitory effect on oxidative damage of endothelial cells.
