Preparation Rapeseed Protein by Double Enzymes

Abstract:

In this manuscript,the determination of optimum conditions of the rapeseed protein hydrolization was performed and the results listed as follows: hydrolyization time 5 hours with alcalase at pH 9.5,55 ℃,3% substrate and enzyme concentration 5500 u/g,the hydrolysis rate could reach to 21.14%,then hydrolization with flavourzyme for 3 hours at pH 6,50 ℃ and enzyme concentration 900 u/g,the bitter value of rapeseed protein hydrolysates was very low under such conditions.The results of decoloration experiment of rapeseed protein hydrolysates with active carbon showed that the optimum conditions were: active carbon dosage 1.5%,pH 4.5,temperature 55 ℃,and reaction time 50 min,the decoloration rate could reach to 32.15%,and the amino acid loss rate was 25.15% under such conditions.