Purification and Refolding Escherichia coli Produced Enterokinase Inclusion Bodies Immobilized by Nickel Chelating Chromatography

Abstract:

A genetic strain E.coli BL21(DE3),which over-expression enterokinase gene,was constructed.When cell density(OD 600) reached at 0.5-0.6 in LB medium which contained 30 mg/L kanamycin,IPTG was feed to the fermentation broth to 0.4 mmol/L and then temperatur